关键词:
大西洋鲑,
虹鳟,
物种鉴定,
循环伏安,
生物传感
Abstract: This study aims to develop new method for species identification of aquatic products,in order to distinguish Atlantic salmon（Salmo salar）adulterated by rainbow trout（Oncorhynchus mykiss）. Based on genomic sequence alignment of Atlantic salmon and rainbow trout,three pairs of polymerase chain reaction（PCR）primers in the genomic region of a nuclear gene myoglobin were designed,which were Atlantic salmon-specific primers,rainbow trout-specific primers,and universal primers for both salmon and trout,respectively. The 5’ ends of forward primers were labeled with biotin,thus the amplification products were able to be captured and anchored on the avidin-modified electrode surfaces. And the 5’ ends of reverse primers were labeled with ferrocene,which made the amplification products electrochemically active and capable to be detected by cyclic voltammetry. The constructed electrochemical bio-sensing system accurately and safely identified whether the samples were from Atlantic salmon or rainbow trout. Moreover,varied samples were applicable,including adult fish,fry,eggs,as well as processed fish products,such as fish paste containing multiple DNA sources. Therefore,the electrochemical bio-sensing detection method established in this study has promising prospects in quality inspection system for aquatic products.
Key words:
Atlantic salmon,
rainbow trout,
species identification,
cyclic voltammetry,
bio-sensing